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. 2020 Sep 29;44(6):2429–2442. doi: 10.3892/or.2020.7783

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Copyright: © He et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Sevoflurane suppresses cell viability and invasion and promotes cell apoptosis by downregulating circ-HMGCS1 in colon cancer cells. (A) The expression of circ-HMGCS1 in SW480 and LOVO cells untreated (control) or treated with 1.7% SEV, 3.4% SEV or 5.1% SEV was determined by RT-qPCR. (B-H) SW480 and LOVO cells were assigned to Control, 5.1% SEV, 5.1% SEV+pcDNA and 5.1% SEV+circ-HMGCS1 groups. (B) The expression of circ-HMGCS1 in SW480 and LOVO cells was examined by RT-qPCR. (C and D) The viability of SW480 and LOVO cells was assessed using MMT assay. (E) The apoptosis of SW480 and LOVO cells was evaluated by flow cytometric analysis. (F) The invasion of SW480 and LOVO cells was examined using Transwell assay. (G and H) The protein levels of cyclin D1, p21 and MMP9 were determined via western blot assay. *P<0.05 vs. the control or the 5.1% SEV+pcDNA group. circ-HMGCS1, circular RNA 3-hydroxy-3-methylglutaryl-CoA synthase 1; SEV, sevoflurane; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; MMP9, matrix metallopeptidase 9; OD, optical density.