Figure 1.

BRDT is a novel binding partner of eIF4EBP1. (A) Experimental workflow diagram. ACHN, 769-P and 768-O cells were cultured and transfected with eIF4EBP1 overexpression plasmids or control plasmids for 48 h. Immunoprecipitation using anti-BRDT or IgG were performed in (B) ACHN, (C) 769-P and (D) 768-O cells, and subsequently subjected to western blotting using the indicated antibodies. (E) ACHN, (F) 769-P and (G) 768-O cells were transiently transfected with pCDNA3.1-BRDT for 36 h, and then, the cells were lysed with NP40 buffer and collected for western blotting using the indicated antibodies. β-actin was used as the loading control. IP, immunoprecipitation; BRDT, bromodomain testis-specific protein; eIF4EBP1, eukaryotic translation initiation factor 4E-binding protein 1.