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. 2020 Oct 8;44(6):2475–2486. doi: 10.3892/or.2020.7796

Figure 2.

Figure 2.

Silencing of BRDT inhibits the growth of renal cell carcinoma and suppresses eIF4EBP1 expression. (A) 293T cells were transiently transfected with two different siRNAs as well as with control siRNA for 48 h. The cells were lysed with NP40 buffer for western blotting with the indicated antibodies. Efficient silencing of BRDT with siBRDT#2 was performed in (B) ACHN, (C) 769-P and (D) 768-O cells for 48 h. The cells were lysed with NP40 buffer for western blotting with the indicated antibodies. β-actin was used as the loading control. (E) ACHN, (F) 769-P and (G) 768-O cells were seeded in six-well plates and transiently transfected with siBRDT#2 for 24 h. The cells were then re-seeded into 96-well plates for another 120 h and subjected to an SRB assay. Points indicate the mean of three experimental repeats. Bars represent the standard deviation. *P<0.05 and **P<0.01 vs. siCon. siRNA, small interfering RNA; SRB, sulforhodamine B; BRDT, bromodomain testis-specific protein; eIF4EBP1, eukaryotic translation initiation factor 4E-binding protein 1; siCon, control siRNA.