Figure 6.

Bromodomain and extraterminal domain inhibitors inhibits renal cell carcinoma tumor growth and c-myc expression in vivo. ACHN cells were inoculated in the subcutaneous regions of nude mice. The mice were treated with 100 mg/kg/day PLX51107 or 90 mg/kg/day INCB054329 or the vehicle for 14 days (n=6 for each group). The (A) volume and (B) weight of ACHN tumors were determined and calculated. (C) Images of the tumors. (D) Total RNA from tumors were isolated and subjected to reverse transcription-quantitative PCR and western blotting using the indicated antibodies to assess c-myc transcription and protein levels. Columns represent the mean of three independent experiments. Bars represent the standard deviation. *P<0.05 and **P<0.01 vs. control. PLX, PLX51107; INCB, INCB054329; Con, control.