Table I.
List of immunoprecipitated proteins.
| IP: Anti-FLAG | IP: Anti-eIF4EBP1 | ||
|---|---|---|---|
| Accession no. | Name | Accession no. | Name |
| NP-001017963.2 | Heat shock protein 90 α family class member 1 | NP-001014431 | AKT serine/threonine kinase 1 |
| NP-002145.3 | Heat shock 70 kDa protein 4 | NP-001158095.1 | Argonaute RISC catalytic component 2 |
| NP-001092.1 | Actin β (ACTB) | NP-997072.2 | Bromodomain testis associated protein |
| NP-003371.2 | Vimentin (Homo sapiens) | NP-004086.1 | Eukaryotic translation initiation factor 4E binding protein 1 |
| AAA91576.1 | α-tubulin | NP-001017963.2 | Heat shock protein 90 α family class member 1 |
| NP-002037.2 | Glyceraldehyde-3-phosphate dehydrogenase | NP-000042.3 | ATM serine/threonine kinase |
ACHN cells were cultured and either transfected with eIF4EBP1 overexpression plasmids or control FLAG plasmids for 48 h. Whole cell lysates were collected for subsequent IP using anti-eIF4EBP1 antibody or anti-FLAG antibody and protein A/G beads. An in-solution trypsin digestion was performed on the IP proteins, and the digested samples were sent for mass spectrometry detection. A representative list of the most abundant proteins identified in the digest indicated bromodomain testis associated protein as a candidate novel binding partner of eukaryotic translation initiation factor 4E binding protein 1. IP, immunoprecipitation.