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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Nat Plants. 2021 Mar 15;7(3):365–375. doi: 10.1038/s41477-021-00876-3

Fig. 4. HPLC elution profiles of pigment extracts from end-point cyclase assays at various AcsF concentrations.

Fig. 4

Assay conditions were 20 µM MgPME, 0.2 mg mL-1 spinach Fd, 0.4 units mL-1 spinach FNR, 2 mM NADPH, 0.29 mg mL-1 catalase and AcsF at various concentrations from 0.23 (1x AcsF) to 3.68 (16x AcsF) µM. Assays were initiated by adding AcsF and terminated after 30 min incubation. Pigment extracts from the assays were analysed by HPLC with elution of pigment species monitored by absorbance at 416 and 440 nm and by fluorescence at 595 and 640 nm with excitation at 440 nm. Insets show the acquired absorbance and fluorescence spectra of MgPME, DV PChlide a, and the potential reaction intermediates, X1 and X2.