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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Nat Plants. 2021 Mar 15;7(3):365–375. doi: 10.1038/s41477-021-00876-3

Extended Data Fig. 2. HPLC elution profiles of pigment extracts from coupled PSI-cyclase assays.

Extended Data Fig. 2

A complete assay contained 2 µM AcsF, 0.04 mg mL-1 spinach Fd, 14 µM MgPME, spinach PSI containing 6 (1x PSI) or 22.4 µM (~4x PSI) Chl a, 20 µM spinach Pc, 2 mM Asc, 60 µM DCPIP and 0.29 mg mL-1 catalase. Assays were incubated either in the dark for 30 min, or under red light illumination for 15 or 30 min. Pigment extracts from the assays were analysed by HPLC and pigment elution was monitored by fluorescence at 640 nm excited at 440 nm. Pigment species were identified by retention times and fluorescence spectra (as in Fig. 4). See Supplementary Fig. 4a for HPLC analysis of pigment extracts from additional control assays.