Extended Data Fig. 3. Targeting of STEEP by CRISPR.

(a) Illustration of gRNAs sequence and location used to target human STEEP. (b) Immunoblot for STEEP in THP-1-derived clones transduced with STING-targeting gRNAs. (c) STEEP KO THP-1 cells were transfected with mRNA encoding WT STEEP. 24 h later, the cells were lysed, and immunoblotted as shown. For comparison, lysates from WT THP-1 cells were included. (d-f) Efficiency of STEEP gene targeting in primary human fibroblasts (d), primary human macrophage (e) and SAVI patient fibroblasts (f). (g) gRNAs used to target STEEP in mice. (h-i) Efficiency of STEEP targeting in MEF cells. The KO scores were calculated based on the ICE Analysis tool from Synthego. In panels b and c, data shown are representative blots from three biologically independent experiments with similar results.