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. Author manuscript; available in PMC: 2021 Jun 14.
Published in final edited form as: Nat Genet. 2020 Dec 14;53(1):100–109. doi: 10.1038/s41588-020-00744-4

Extended Data Fig. 9. The role of transcription factors in the recruitment of cohesin.

Extended Data Fig. 9

a-c, heatmaps showing the binding of pluripotency transcription factor SOX2 (a), OCT4 (b) and NANOG (c) remain unchanged following WAPL depletion. d, A published OCT4-FKBP-mCherry cell lines was used. The FKBP degron can be degraded with the dTAG-13 molecule. e, A western blot shows protein levels of OCT4 after 6 and 24 hours of dTAG-13 treatment (cropped). f-g, ChIP-seq heatmaps showing MED1 binding at OCT4 binding sites and NANOG binding sites in the OCT4-FKBP (f) and NANOG-FKBP (g) lines, respectively. h-i, ChIP-seq heatmaps showing RAD21 binding and chromatin accessibility at CTCF binding sites in OCT4-FKBP (h), SOX2-FKBP (i, left) and NANOG-FKBP (i right). For OCT4-FKBP CTCF sites from V6.5 mESCs were used for alignment and for Nanog-FKBP, which are derived from E14 mESCs CTCF sites identified in WAPL-AID cells were used.