Extended Data Fig. 10. Further characterization of RAD21 binding in WAPL-AID NPCs.

a, Staining for Nestin (Green) and GFAP (red) in NPCs derived from the WAPL-AID cell line (representative images from 2 experiments and 3 separate fields per experiment). b, Staining for alkaline phosphatase in NPCs derived from the WAPL-AID cell line (n=1). c, Comparison of the differential genes between ESC and NPC identified in a published study and in the WAPL-AID line. d, Venn diagram shows overlap of RAD21 binding sites measured ChIP-seq for untreated and 24h treated WAPL-AID NPCs. Only sites that showed consistent unique or shared binding across two replicates were included. Peak calling was performed on a subset of 7 M sequencing reads for all the samples. e, MA-plot showing the log₂ fold-change as a function of the number of reads in a RAD21 peak. Highlighted dots indicate significantly changed RAD21 levels based on DESeq2 with an FDR <0.01 and a fold-change of at least 2 up or down. Red is increase in RAD21, blue is decrease. f, Annotation of the motifs identified in Fig. 5d.