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. Author manuscript; available in PMC: 2021 Jun 14.
Published in final edited form as: Nat Genet. 2020 Dec 14;53(1):100–109. doi: 10.1038/s41588-020-00744-4

Fig. 4. Pioneer factors OCT4 and SOX2 create an open chromatin platform for cohesin binding.

Fig. 4

a, heatmaps showing RAD21 level and chromatin accessibility over OCT4 binding sites following 24 h of OCT4 depletion. b, Example locus containing the Esrrb gene shows RAD21 binding and chromatin accessibility over RDCs in treated and untreated OCT4-FKBP cells. OCT4 ChIP-seq track shows previously published OCT4 profiles in the same cell line34. c, Two FKBP-tagged cell lines were generated, a SOX2-FKBP-mCherry line and a NANOG-FKPB-eGFP (see Methods for details on construction). The FKBP degron can be degraded with the dTAG-13 molecule35. d, Western blots show protein levels of SOX2 (left) and NANOG (right) after 6 and 24 hours of dTAG-13 treatment (cropped, n = 1). e, Similar heatmaps as (a), but over SOX2 binding sites following SOX2 depletion. f, Similar heatmaps as (a,e), but over NANOG binding sites following NANOG depletion.