Extended Data Fig. 6. The movement of POTRA5 towards the periplasmic exit of the lumen of the BamA barrel could push RcsF upwards.

(a,b) Lateral view of the initial and final conformations, respectively, of the BamA-RcsF complex during the dynamic importance sampling simulation (DIMS) of the BamA-RcsF complex. (c,d) Bottom view (from the periplasm) of the above conformations. BamA and RcsF are colored in orange and blue, respectively. The initial conformation of the system (BamA and RcsF) corresponds to the structure determined in this work (PDB code 6T1W)⁵ with the POTRA1-4 domains removed. The final conformation of BamA is similar to the outward-open structure (PDB code 5D0Q). The explicit outer membrane and solvent are not shown for clarity. (e) Expression from BamA^hinge from a plasmid in ΔbamA cells leads to a severe growth defect when cells are grown at 37°C in rich media, but not when they are grown in minimal media at 30°C. Cells were grown in M9 minimal glucose medium at 30°C until they reached OD₆₀₀ =1. Tenfold serial dilutions were made in M9 minimal glucose, plated onto M9 minimal glucose or LB agar, and incubated at 30°C or 37°C. Plates were supplemented with ampicillin (200μg/ml). n=3 biologically independent experiments.