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. Author manuscript; available in PMC: 2021 Mar 19.
Published in final edited form as: Nat Chem Biol. 2020 Jun 22;16(9):1019–1025. doi: 10.1038/s41589-020-0575-0

Extended Data Fig. 1. RcsF can be co-purified with the BAM complex.

Extended Data Fig. 1

(a,b,c) Gel filtration profiles of the affinity-purified BamAB-RcsF, BAM-RcsF and BAM complexes. The size exclusion chromatography was performed using a HiLoad 10/300 Superdex 200pg. The input and peak fractions were collected and the samples were analyzed by blue native electrophoresis with Coomassie staining. The migration pattern of BamABCDE-RcsF (b) was modified compared to BamABCDE (c) upon size exclusion chromatography (band 8 increases), reflecting the higher instability of the BamABCDE-RcsF complex. n= 4 biologically independent experiments.