Extended Data Fig. 4. LIFR downregulation enables enrichment from WT C57BL/6 mice of the pDCs engaged in IFN-I.

pDCs were sorted from 36h MCMV-infected WT C57BL/6 mice, with a protocol including an enrichment of LIFR^lo cells to increase the capture efficiency for pDCs engaged in IFN-I production. a, Monocle pseudo-temporal analysis showing bifurcation of the inferred pDC activation trajectory in two major branches, Y53 and Y50. b, Expression of the IFN-I meta-gene along pseudo-time for the Y53 (top) and Y50 (bottom) branches of the pDC activation trajectory. c, LIFR expression along pseudo-time on the pDCs from the common root (empty thin orange circles), Y53 branch (filled orange triangles) and Y50 branch (empty thick dark red circles) of the pDC activation trajectory. d, Expression of individual genes along pseudo-time for the cells from the common root (empty thin orange circles), Y53 branch (filled orange triangles) and Y50 branch (empty thick dark red circles) of the pDC activation trajectory. A polynomial curve was fit to the data for each of the three segments of the trajectory.