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. Author manuscript; available in PMC: 2021 Mar 28.
Published in final edited form as: Nat Cell Biol. 2020 Sep 28;22(10):1180–1186. doi: 10.1038/s41556-020-00584-8

Extended Data Fig. 7. Effect of perturbations to mitochondrial dynamics on MRG distribution.

Extended Data Fig. 7

Representative widefield-microscopy images of HeLa cells silenced using siRNAs against Drp1 (second row) or Mfn2 (third row). Cells were fixed after 72 hours of silencing. A negative control siRNA was used in parallel, and is shown in the first row. MRGs and nucleoids were immunolabeled using anti-FASTKD2 (green) and anti-DNA (magenta), respectively. Mitochondria were labelled using Mitotracker Deep Red staining (cyan) (The experiment has been performed twice with similar results). Scale-bar: 10 μm. Disrupted MRG and nucleoid positioning, and clumped appearance as described for mitobulbs is apparent when either Drp1 and Mfn2 are silenced, but the negative control shows well-dispersed MRGs and nucleoids.