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. Author manuscript; available in PMC: 2021 May 2.
Published in final edited form as: Nat Methods. 2020 Nov 2;17(12):1254–1261. doi: 10.1038/s41592-020-00989-1

Figure 1. System design.

Figure 1

(A) Molecular structure of BoNT/A (PDB id 3BTA, [40]). Toxin domains are colored red (protease), yellow (belt), green (Translocation, TD) and blue (Receptor Binding, RBD). (B) Schematic showing the steps leading to labelling. (C) Retargeted BoNT/A: the RBD from (A) is replaced by an anti-GFP nanobody (blue). Also shown in light green the bound GFP (PDB id 3OGO, [13]). (D) Proteins and molecular mechanisms mediating each step from (B). Colors as in (C) except pink sections in BoNT/A::GFPnb::CD2 which indicate TEV cleavage sites. * The release step was shown to be TEV independent later in the project.