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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Algal Res. 2021 Mar 5;55:102255. doi: 10.1016/j.algal.2021.102255

Fig. 2. Ketocarotenoids accumulation in transformed lines.

Fig. 2

a) orange color phenotype of transformed lines in UVM4 (UV-mediated mutant 4) background expressing FtOCP (Orange Carotenoid-binding Protein from Fischerella ther-malis) fused to YFP (Yellow Fluorescent Protein) alone or in addition to CrBKT (β-carotene ketolase from Chlamydomonas reinhardtii) fused to spectinomycin resistance (AadA, aminoglycoside-3”-adenylyltransferase gene), indicated respectively as UVM4 + OCP_YFP or UVM4 + OCP_YFP + BKT_AadA. b) Ketocarotenoids accumulation expressed as arbitrary units (a.u.) for transformed lines expression both FtCOP and CrBKT (ocp1_bkt1–12 and ocp2-bkt1–12) compared to the background strain UVM4. c) Representative HPLC of pigments from UVM4 (black) ocp (orange) and ocp-bkt (red) strains. Chromatograms are shown reported the absorbance (ABS) of the different peaks at 440 nm. 1: neoxanthin; 2: loroxanthin; 3: violaxanthin; 4: antheraxanthin; 5: lutein; 6: zeaxanthin; 7: chlorophyll b; 8: chlorophyll a; 9 β-carotene; 10,11, 12: astaxanthin; 13: canthaxanthin. The data reported are representative of three independent experiments.