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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Algal Res. 2021 Mar 5;55:102255. doi: 10.1016/j.algal.2021.102255

Fig. 3. Purification of OCP proteins.

Fig. 3

a) red/orange color of purified OCP (Orange Carotenoid-binding Protein) proteins isolated from Chlamydomonas reinhardtii strains expressing recombinant OCP protein (OCP) or both BKT (β-carotene ketolase) and OCP (OCP-BKT); b) Western blot analysis performed by using specific antibody recognizing YFP (Yellow Fluorescent Protein) fused to OCP proteins in in isolated fractions after affinity chromatography (OCP and OCP-BKT) and in total protein extracts from the background strains UVM4 and from the transformant strains ocp1, ocp1-bkt1 and bkt1 expressing respectively OCP, OCP and BKT or BKT only; c) absorption spectra of isolated proteins reported as optical density (OD); d) fluorescence emission spectra of OCP proteins purified from ocp1 (OCP), ocp1-bkt1 (OCP-BKT) and YFP expressing lines reported as arbitrary units (a.u.); e) composition of carotenoids bound by OCP and OCP-BKT isolated proteins. Data are reported in percentage of total carotenoids. NEO: neoxanthin; CANTHA: canthaxanthin; LORO: loroxanthin; LUTEIN: lutein; ASTA: astaxanthin. f) percentage of refolded FtOCP holoproteins compared to total FtOCP isolated polypeptides. Error bars are reported as standard deviations (n = 3).