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. Author manuscript; available in PMC: 2021 Mar 26.
Published in final edited form as: Nat Biotechnol. 2020 Jul 6;38(12):1415–1420. doi: 10.1038/s41587-020-0570-8

Figure 2. CsgG:CsgF cryo-EM structure reveals a dual constriction pore.

Figure 2

(a) Close-up ribbon and sticks (CsgF) representation of a single CsgG (gold) and CsgF (purple) protomer of the CsgG:CsgF cryo-EM structure. The CsgG constriction formed by Y51, N55 and F56 is highlighted magenta, and the N-terminal four residues and the conserved NPXFGG motif in CsgF are highlighted in cyan. Oxygens are red, nitrogens blue. H-bonds anchoring the CsgG:CsgF interaction are depicted as dashed red lines. (b, c, d, e) The CsgG:CsgF pore shown in side (b), top (c) and cross-sectional views (d, e), depicted in ribbon (b, c and e) and solvent-accessible surface (d) representations, coloured as in panel a. GC: CsgG-constriction, FC: CsgF-constriction. Only the CsgF N-terminus (residue G1 to P35) forming the CsgF-constriction peptide (FCP) could be resolved in the cryo-EM density (Extended Data Figure 2).