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. Author manuscript; available in PMC: 2021 Jul 4.
Published in final edited form as: Nat Microbiol. 2021 Jan 4;6(2):196–208. doi: 10.1038/s41564-020-00816-5

Extended Data Fig. 7. Kefir grain growth profits from rare species and supplements.

Extended Data Fig. 7

Different kefir species were supplemented to the UHT-milk used for kefir propagation in this study (Methods). This suspension was then used as a medium to grow kefir grains in. The gain in wet-weight after 3 passages (circa 1 week) was then compared to kefir grains grown in milk and milk supplemented with proteinase K and yeast extract, respectively. Compared to the negative control, many rare species and few main kefir species supported the growth of the kefir grain. However, the effect of addition of proteinase K and yeast extract to the milk medium had the biggest effect on grain growth. Significance estimated by using two-sided t-test, p-values: * <0.05, ** <0.01, *** <0.001. N=4 biologically independent samples, data are presented as mean values +/- SD. P-values: L. mesenteroides, 0,045; L. lactis (SB-150), 0,00034; S. haemolyticus, 0,0026; R. dentocariosa, 0,0012; Rhodotorula, 0,033; proteinase K, 0,00018; yeast extract, 8,83351E-07.