Extended Data Fig. 4. LTβR signaling in Cxcl13-Cre⁺ cells governs BRC maturation.

a,b, Representative immunofluorescence images of EYFP, TdTom and B220 staining in lymph nodes (a) and regions underpinning B cell aggregates (b) in naive Cxcl13-Cre/TdTom Ltbr ^fl/fl mice (Ltbr ^fl/fl). c, Enumeration of TdTom⁺ EYFP⁺ cells per lymph node (LN) in naive Cxcl13-Cre/TdTom control or Ltbr ^fl/fl mice. d, Quantification of the mean fluorescence intensity (MFI) of TdTom on EYFP⁺ cells from naive control or Ltbr ^fl/fl mice. e, Representative flow cytometry plots of MAdCAM1 and CD21/35 on TdTom⁺ EYFP⁺ cells from naive Ltbr ^fl/fl mice. Mean percentages and SEM are indicated. N = 14 mice, 4 independent experiments. f,g, Representative immunofluorescence images of EYFP, TdTom and B220 staining in lymph nodes (f) and regions underpinning B cell aggregates (g) in day 12 VSV-immunized Ltbr ^fl/fl mice. h, Enumeration of TdTom⁺ EYFP⁺ cells per lymph node in immunized control or Ltbr ^fl/fl mice. i, Quantification of the MFI of TdTom on EYFP⁺ cells from lymph nodes of immunized control or Ltbr ^fl/fl mice. j, Representative flow cytometry plots of MAdCAM1 and CD21/35 on TdTom⁺ EYFP⁺ cells from immunized Ltbr ^fl/fl mice. Mean percentages and SEM are indicated. N = 13 Ltbr ^fl/fl mice, 5 independent experiments. (a,b,f,g) Images are representative of 6 Ltbr ^fl/fl per condition. (c,d) N = 11 control mice and 13 Ltbr ^fl/fl mice; 4 independent experiments. (h,i) N = 16 control mice and 11 Ltbr ^fl/fl mice, 4 independent experiments. (c,d,h,i) Mean and SEM are indicated. P values as per two-tailed Mann Whitney test.