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. Author manuscript; available in PMC: 2021 Mar 29.
Published in final edited form as: J Mol Biol. 2021 Feb 19;433(8):166878. doi: 10.1016/j.jmb.2021.166878

Figure 3:

Figure 3:

SDS PAGE gel electrophoresis of α-syn incubated with various concentrations of Brazilin (0x, 1x, and 10x) at 0 h (a), 24 h (b), 72 h (c), and 168 h (d). The T represents total, S represents supernatant taken after ultracentrifugation at 100,000 xg at 4°C for 30 minutes, and P represents the pellet after ultracentrifugation after a wash with 1x PBS resuspended in an equal buffer volume. e) Densitometric quantification of SDS-PAGE bands from supernatant (S) and pellet (P) fractions. Total (T) fractions from untreated α-syn samples from each gel were set as 100% and mean densities and SD were calculated from these data (n = 4).