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. Author manuscript; available in PMC: 2021 Mar 30.
Published in final edited form as: Nat Chem Biol. 2020 Aug 10;17(1):30–38. doi: 10.1038/s41589-020-0611-0

Figure 3. Cell cycle sensors based on greenFAST and redFAST.

Figure 3

Zebrafish embryos were injected with redFAST-zGem(1-100)-P2A-greenFAST-zCdt1(1-190) mRNA at one-cell stage, and time-lapse imaging was performed starting from 256-cell stage on embryos incubated with 5 μM HMBR and 5 μM HBR-3,5DOM. (a) Representative timelapse (n = 3 from 3 independent embryos) of single cells at high magnification. Scale bar 20 μm. (b) Corresponding quantification of fluorescence signal over time (cells with white arrows in the panel a). (c) Representative timelapse (n = 3 from 3 independent embryos) of the whole embryo (see also Supplementary Video 2). Scale bar 100 μm.