. Author manuscript; available in PMC: 2021 Mar 30.

Published in final edited form as: Nat Chem Biol. 2020 Aug 10;17(1):30–38. doi: 10.1038/s41589-020-0611-0

Table 1. Physicochemical characteristics of FAST, iFAST, greenFAST, and redFAST with HMBR and HBR-3,5DOM in PBS pH 7.4.

Abbreviations are as follows: λ_abs, wavelength of maximal absorption; λem, wavelength of maximal emission; ε, molar absorptivity at λ_abs; ϕ, fluorescence quantum yield; K _D thermodynamic dissociation constant.

			HMBR					HBR-3,5DOM			K _D,HMBR/
											K _D,HBR-3,5DOM
Protein	K _D	λ_abs	λ_em	ϕ	ε	K _D	λ_abs	λ_em	ϕ	ε
	(μM)	(nm)	(nm)		(mM^–1cm^–1)	(μM)	(nm)	(nm)		(mM^–1cm^–1)
FAST^7,8	0.13	481	540	0.23	45	0.97	516	600	0.31	39	0.1
iFAST⁹	0.07	480	541	0.22	41	0.41	516	600	0.40	38	0.2
greenFAST	0.09	478	544	0.23	40	16.2	516	597	~0.29	n.d.	0.005
redFAST	12	502	554	~0.18	n.d.	1.3	556	603	0.29	43	9.2

n.d. not determined