Extended Data Fig. 9. Total nuclear accumulation of SiR-PyPDS and SiR-iPyPDS in U2OS cells.

Total fluorescence intensity measured inside the nuclei of >300 U2OS cells after incubation with 10μM SiR-PyPDS or SiR-iPyPDS by standard confocal microscopy at 633 nm. Each point on the graph represents the total fluorescence of SiR measured at 633 nm per nuclei, data are plotted as the mean of >300 nuclei measured in 3 independent replicates. Total fluorescence measurement revealed comparable ability of the two molecules to accumulate in the nuclei. Error bars indicate mean ± sd.