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. Author manuscript; available in PMC: 2021 Mar 30.
Published in final edited form as: Nat Microbiol. 2020 May 11;5(8):1016–1025. doi: 10.1038/s41564-020-0716-y

Extended Data Fig. 7. RagAB and RagB bind peptides selectively.

Extended Data Fig. 7

MST titration curves with the P4-FAM peptide for (a) RagAB from ATCC 33277, (b) RagAB from W83 and (c) Omp40-41 from W83 (negative control). The other panels show MST profiles for unlabelled P21, P12 or P4 binding to His-tag labelled W83 RagAB (d-f) and His-tag labelled W83 RagB (g-i). Experiments and listed Kd values represent the mean of three independent experiments ± SD.