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. Author manuscript; available in PMC: 2021 Mar 30.
Published in final edited form as: Nat Microbiol. 2020 May 11;5(8):1016–1025. doi: 10.1038/s41564-020-0716-y

Figure 3. DPeptide and plug dynamics in RagA2 B 2 revealed via cryo-EM.

Figure 3

a, Density maps viewed from the membrane plane of the closed-closed, open-closed and open-open RagA2B2 complexes coloured as in Figure 2. b, c. Isolated density for the plug domain and the bound peptide ensemble viewed as in (a) and after a 90 degree clockwise rotation (c). Additional density for the plug domains of open RagAB complexes is shown in green. d,e, Superposition of RagA plug domains in the open (yellow) and closed (cyan) RagAB complexes of the OC state, showing different dynamics (d;view approximately as inc) and conformational changes (e) in going from the open to the closed state. The Ton box region Q103-G108, visible only in the open state, is coloured black with electron density shown in (d). In e, density for open and closed complexes is shown for the region I214-R218.