Extended Data Fig. 7. Monosynaptic rabies tracing identifies Chx10 Gi presynaptic inputs.

a, A rabies transsynaptic tracing approach was used to identify presynaptic inputs to Chx10 Gi neurons (see also, Fig. 7 and Supplementary Table 2). b, Injection site in rostral Gi. Initial site of infection is visualized as a large population of mCherry⁺ neurons, accompanied by dense mCherry⁺ processes. Starter neurons of the ipsilateral Gi do not exhibit a strong input from neurons of the contralateral Gi. c, Quantification of mCherry-labeled neurons in the ipsilateral and contralateral Gi. ***P = 0.0002, paired two-tailed t-test, n = 6 mice from one experiment. d, Bilateral input to Chx10 Gi neurons from neurons of primary motor and somatosensory cortex. Open yellow triangles point to soma from pyramidal neurons. Yellow asterisk indicates an apical dendrite. e, Quantification of mCherry-labeled neurons in the ipsilateral and contralateral cortex. ***P = 0.001, paired two-tailed t-test, n = 6 mice from one experiment. f, Input to Chx10 Gi neurons from neurons of the ipsilateral zona incerta. Presynaptic neurons were observed primarily in the dorsal (ZID) and caudal aspects of the zona incerta. g, Quantification of mCherry-labeled neurons in the ipsilateral and contralateral zona incerta. ***P < 0.001, paired two-tailed t-test, n = 6 mice from one experiment. h, Unilateral input to Chx10 Gi neurons from the ipsilateral mesencephalic reticular formation. i, Quantification of mCherry-labeled neurons in the ipsilateral and contralateral mesencephalic reticular formation. ***P = 0.001, paired two-tailed t-test, n = 6 mice from one experiment. j, Medial (Med) and lateral (Lat) deep cerebellar nuclei exhibited unilateral or bilateral, respectively, input to Chx10 Gi neurons. k, Quantification of mCherry-labeled neurons in the deep cerebellar nuclei. Med, **P = 0.0024, paired two-tailed t-test, n = 6 mice from one experiment; Lat, P = 0.69, paired two-tailed t-test, n = 6 mice from one experiment. Box-and-whisker plots in (c,e,g,i,k) give the median, 25^th and 75^th percentiles, and range.