Extended Data Fig. 4. Fate-mapping of perivascular lymphoid tissue organizer cells.

a, Schematic description of fate-mapping experiments in Ccl19 ^iBbw2.1 mice. Pregnant mice were treated with Doxycyclin (Dox) at embryonic day (E) 18.5 and offsprings were analysed at indicated timepoints. b, Images showing PP anlage at E18.5 harvested from Ccl19 ^iBbw2.1 embryos. Dashed line indicates intestinal epithelium. c, Images showing PP anlagen at postnatal day (P) 1 and P4 (in d) harvested from Ccl19 ^iBbw2.1 pups kept on Dox since E18.5. Arrowheads indicate perivascular EYFP⁺ cell cluster. e, Images showing PP anlage at P7 harvested from Ccl19 ^1Bbw2.¹ pups kept on Dox since E18.5. Boxed area indicates representative area of higher magnification images below, Arrowheads indicate EYFP⁺ cell cluster. f, Images show whole-mount analysis of adult PP from Ccl19 ^1Bbw2.¹mice, which were kept without (w/o) Dox. g, Images depicting histological section of adult PP harvested from Ccl19 ^1Bbw2.1mice kept on doxycycline since E18.5. h, Images showing high resolution confocal analysis of EYFP⁺ and tdTOMATO⁺ cell clusters in PP from adult Ccl19 ^iBbw2.1 mice kept on Dox since E18.5.Scale bars, 20 μm (b,c), 50 μm (d), 100 μm (e), 500 μm (f,g), 10 μm (h). Images are representative of at least 3 mice from 2-3 independent experiments.