Extended Data Fig. 2. Single cell RNA sequencing analysis of PP FRC.

a, Flow cytometry based sorting strategy for PDPN⁺VCAM1⁺ fibroblasts from adult Ccl19 ^eYFP and Col6a1 ^eYFP PPs. b, Statistics for cell sorting and scRNA sequencing. c, UMAPs showing all fibroblasts sequenced, after removal of contaminating cells (see Materials and Methods). Highlighted are EYFP-expressing cells from the two different Cre driver lines, small UMAPs show gene expression to determine Adamdec1 ⁺ lamina propria fibroblasts (LPF) and Ccl19 ⁺ PP FRCs. d, UMAPs displaying 12 clusters of re-embedded EYFP⁺ fibroblasts and the contribution of the two Cre driver lines is shown in the boxed split UMAPs. e, Gene expression feature UMAPs showing Adamdec1 expression to determine lamina propria fibroblasts, Bmp5 to determine subepithelial fibroblasts (SEF) and Clu for the identification of PP FRCs among EYFP expressing cells. f, Images showing PP from adult Ccl19 ^eYFP mice. g, Heatmap displaying top marker gene expression of individual cells for each PP FRC cluster. Meso (Mesothelial cells), Myo (Myocytes); Scale bar, 500 μm. Images are representative of 3 mice. ScRNA-sequencing data represents 9719 fibroblasts (c) or 4492 EYFP⁺ fibroblasts (d), 8 biological replicates, 4 independent experiments.