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. Author manuscript; available in PMC: 2021 Apr 7.
Published in final edited form as: Anal Chem. 2016 Jan 6;88(2):1222–9. doi: 10.1021/acs.analchem.5b03513

Figure 1. Microfluidic cell culturing workflow.

Figure 1

(A) Layout of the microfluidic device, consisting of a 4-by-6 array of interconnected drops with a single inlet and single outlet. Liquid is loaded from the top and automatically distributed all over the network due to capillary forces. (B) Single-cell loading into standing drops by using FACS and spheroid loading by using pipets. (C) Culturing under controlled perfusion and a close-up of the outlet: state 1, larger drop = liquid removal; state 2, smaller drop = no liquid outflow. (D) Parallel cell and spheroid transfer from the hanging-drop network to a receiver plate.