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. Author manuscript; available in PMC: 2021 Apr 7.
Published in final edited form as: Anal Chem. 2016 Jan 6;88(2):1222–9. doi: 10.1021/acs.analchem.5b03513

Figure 5.

Figure 5

(A) Phase contrast images (10×) of all 24 drops at day 1, after loading 50 granulocyte/macrophage progenitor (GMP) cells from bone marrow suspension into each drop using a FACS unit, and at day 5 after subsequent culturing in the hanging drops (target drops are schematically marked in black, scale bar is 200 μm). (B) Flow-cytometric characterization of retrieved cells after 5 days in culture. (C) Yellow fluorescence micrographs of the hanging-drop culture taken every day (scale bar is 200 μm, see Supplementary Movie 2 for full time-lapse sequence).