Extended Data Table 1. Complete list of S. pombe strains used in this study.
List of all the Schizosaccharomyces pombe strains used in this study along with their full genotypes and any relevant strain construction notes.
| Strain | Genotype | Reference | Strain construction details |
|---|---|---|---|
| SO4913 | cut11-GFP:ura4+ pBiP1-mCherry-AHDL:leu1+ h+ | Snezhana Oliferenko lab | For original AHDL 32 |
| SO6600 | pBiP1-NLS-GFP-NLS:leu1+_ade-_leu1-32_ura4D-18 h- | Snezhana Oliferenko lab | Integration at leu1 locus |
| MBY5861 | cut11-mCherry:ura4+ h- | Mohan Balasubramanian lab | |
| MBY6659 | pAct1 Lifeact-GFP:leu+ atb2-mCherry:Hph leu1-32 ura4-D18 h- | Mohan Balasubramanian lab | pCDL1484 integrated into MBY5856 |
| SI235 | Hph<<ark1-as3 h- | Silke Hauf lab | Hph integrated 390 bp upstream of Ark1 start codon; mutations are L166A (gatekeeper), S229A Q28R Q176R (suppressor) |
| SI236 | Hph<<ark1-as3 h+ | Silke Hauf lab | Hph integrated 390 bp upstream of Ark1 start codon; mutations are L166A (gatekeeper), S229A Q28R Q176R (suppressor) |
| PN1 | 972 h-(wild type) | Paul Nurse lab | |
| PN2 | 972 h+ (wild type) | Paul Nurse lab | |
| GD111 | Hph<<ark1-as3 atb2-mCherry:Hph h? | This study | MBY6659 X SI236 |
| GD121 | cut11-mNeonGreen:Kan Hph<<ark1-as3 atb2-mCherry:Hph h? | This study | cut11-mNeonGreen:Kan transformed into GD111 |
| GD130 | les1-mNeonGreen:Kan Hph<<ark1-as3 atb2-mCherry:Hph h? | This study | les1-mNeonGreen:Kan transformed into GD111 |
| GD138 | les1-mNeonGreen:Kan h+ | This study | les1-mNeonGreen:Kan transformed into 972 h+ |
| GD141 | les1-mNeonGreen:Kan atb2-mCherry:Hph h? | This study | GD138 X MBY6659 |
| GD155 | Hph<<ark1-as3 pBiP1-mCherry-AHDL::leu1+ h+ | This study | SO4913 X SI235 |
| GD171 | les1::Hph cut11-mCherry:ura4+ h- | This study | les1::Kan transformed into MBY5861 |
| GD172 | les1-mNeonGreen:Kan cut11-mCherry:ura4+ h+ | This study | GD138 X MBY5861 |
| GD173 | nup60-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | nup60-mNeonGreen:Kan transformed into MBY5861 |
| GD175 | nup60-mNeonGreen:Kan Hph<<ark1-as3 atb2-mCherry:Hph h? | This study | nup60-mNeonGreen:Kan transformed into GD111 |
| GD176 | nup60-mNeonGreen:Kan les1::Hph cut11-mCherry:ura4+h- | This study | nup60-mNeonGreen:Kan transformed into GD171 |
| GD206 | les1::Hph pBiP1-NLS-GFP-NLS:leu1+_ade-_leu1-32_ura4D-18 h- | This study | les1::Hph transformed into SO6600 |
| GD220 | alm1-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | alm1-mNeonGreen:Kan transformed into MBY5861 |
| GD224 | nup211-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | nup211-mNeonGreen:Kan transformed into MBY5861 |
| GD225 | pom34-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | pom34-mNeonGreen:Kan transformed into MBY5861 |
| GD227 | cdc15-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | cdc15-mNeonGreen:Kan transformed into MBY5861 |
| GD229 | nup60-mEOS3.2:Kan h+ | This study | nup60-mEOS3.2:Kan transformed into 972 h+ |
| GD250 | nup60-mCherry:Kan h+ pBiP1-NLS-GFP-NLS:leu1+_ade-_leu1-32_ura4D-18 h- | This study | nup60-mCherry:Kan transformed into SO6600 |
| GD253 | les1::Hph nup60-mCherry:Kan h+ pBiP1-NLS-GFP-NLS:leu1+_ade-_leu1-32_ura4D-18 h- | This study | les1::Hph transformed into GD250 |
| GD255 | les1-mNeonGreen:Kan Hph<<ark1-as3 pBiP1-mCherry-AHDL::leu1+ h+ | This study | les1-mNeonGreen:Kan transformed into GD255 |
| GD257 | cta4-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | cta4-mNeonGreen:Kan transformed into MBY5861 |
| GD259 | nup60-mCherry:Hph les1::NatR cmp7-mNeonGreen:Kan h- | This study | nup60-mCherry:Hph transformed into GD273 |
| GD261 | nup120-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | nup120-mNeonGreen:Kan transformed into MBY5861 |
| GD263 | nup82-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | nup82-mNeonGreen:Kan transformed into MBY5861 |
| GD264 | nup37-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | nup37-mNeonGreen:Kan transformed into MBY5861 |
| GD265 | nup44-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | nup44-mNeonGreen:Kan transformed into MBY5861 |
| GD266 | nup40-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | nup40-mNeonGreen:Kan transformed into MBY5861 |
| GD270 | nem1::Hph nup211-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | nem1::Hph transformed into GD224 |
| GD273 | les1::NatR cmp7-mNeonGreen:Kan h- | This study | les1::NatR transformed into KG18766 |
| KG18766 | cmp7-mNeonGreen:Kan h- | Kathy Gould lab | cmp7-mNeonGreen:Kan transformed into 972 h- |
| GD275 | nem1::Hph les1-mNG:kanR cut11-mCh:ura4+ h+ | This study | nem1::Hph transformed into GD172 |
| GD181 | imp1::Kan les1::Hph cut11-mCherry:ura4+ h- | This study | imp1::Kan transformed into GD171 |
| GD139 | les1:mScarlet:Hph h+ | This study | les1:mScarlet:Hph transformed into 972 h+ |
| GD140 | cmp7-mNeonGreen:Kan les1:mScarlet:Hph h+ | This study | KG18766 crossed with GD139 |
| GD283 | les1(1-204):mNeonGreen cut11-mCherry:ura4+ h- | This study | les1(1-204):mNeonGreen transformed into MBY5861 |
| GD284 | les1(1-291):mNeonGreen cut11-mCherry:ura4+ h- | This study | les1(1-291):mNeonGreen transformed into MBY5861 |
| GD285 | les1(1-94):mNeonGreen cut11-mCherry:ura4+ h- | This study | les1(1-94):mNeonGreen transformed into MBY5861 |
| GD290 | imp1::Hph alm1-mNeonGreen:Kan cut11-mCherry:ura4+ h- | This study | imp1::Hph transformed into GD220 |
| GD292 | imp1::Hph les1-mNeonGreen:Kan cut11-mCherry:ura4+ h+ | This study | imp1::Hph transformed into GD172 |