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. Author manuscript; available in PMC: 2021 Apr 10.
Published in final edited form as: Nature. 2021 Feb 5;592(7853):277–282. doi: 10.1038/s41586-021-03291-y

Figure 4. Spike mutant D796H + ΔH69/V70 infectivity and sensitivity convalescent plasma (CP).

Figure 4

A. western blot of virus pellets after centrifugation of supernatants from cells transfected with lentiviral pseudotyping plasmids including Spike protein. Blots are representative of two independent transfections. B. Single round Infectivity of luciferase expressing lentivirus pseudotyped with SARS-CoV-2 Spike protein (WT versus mutant) on 293T cells co-transfected with ACE2 and TMPRSS2 plasmids. Infectivity is corrected for reverse transcriptase activity in virus supernatant as measured by real time PCR. Data points represent technical replicates (n=3) with mean and error bars representing standard error of mean; data are representative of two independent experiments C-E. convalescent plasma (CP units 1-3) neutralization potency against pseudovirus virus bearing Spike mutants D796H, ΔH69/V70 and D796H + ΔH69/V70 F, G patient serum neutralisation potency against pseudovirus virus bearing Spike mutants D796H, ΔH69/V70 and D796H + ΔH69/V70. Patient serum was taken at indicated Day (D). Indicated is serum dilution required to inhibit 50% of virus infection (ID50), expressed as fold change relative to WT. Data points represent means of technical replicates and each data point is an independent experiment (n=2-6). Mean of data points in C-G is shown by horizontal bars.