. Author manuscript; available in PMC: 2021 Apr 14.

Published in final edited form as: Mol Pharmacol. 2017 Aug 7;92(4):401–413. doi: 10.1124/mol.117.108688

Table 1. List of forward and reverse primers used for generation of mutants.

Mutations		Primers
M584E	Forward	5′-agattctgcttttggacgaggccacctcagctctgg-3’
	Reverse	5′-ccagagctgaggtggcctcgtccaaaagcagaatct-3’
E502S	Forward	5′-agatcagattgggatagtgtcgcaagagccagttctgttc-3’
	Reverse	5′-gaacagaactggctcttgcgacactatcccaatctgatct-3’
R1221G and E1223Q	Forward	5′-aggggtctcaactctctggagggcagaaacaacgc-3’
	Reverse	5′-gcgttgtttctgccctccagagagttgagacccct-3’
K461M	Forward	5′-ggacccagtggagctggaatgagtacagcactgcaac-3’
	Reverse	5′-gttgcagtgctgtactcattccagctccactgggtcc-3’
K1119M	Forward	5′-ttgttgggagcagtggatgtggcatgagcactagcattc-3’
	Reverse	5′-gaatgctagtgctcatgccacatccactgctcccaacaa-3’
D590A	Forward	5′-ccacctcagctctggccaatgagagtgaagc-3’
	Reverse	5′-gcttcactctcattggccagagctgaggtgg-3’
D1250A	Forward	5′-agccacttctgccttagccacagaaagtgaaaagac-3’
	Reverse	5′-gtcttttcactttctgtggctaaggcagaagtggct-3’
E1244Q	Forward	5′-cgagatcctaaaatcttgctactagatcaggccacttctgcctt-3’
	Reverse	5′-aaggcagaagtggcctgatctagtagcaagattttaggatctcg-3’