Figure 3. Adaptive sequencing enriching for the least abundant genome with centrifuge read classification and ensuring uniform 50x coverage.

A) Mean read lengths for reads sequenced from the ZymoBIOMICS mock metagenomic community mapped against the provided references. Read lengths are reported for the whole run, the deliberately sequenced reads and those which were actively unblocked. B) Shows cumulative coverage of each ZymoBIOMICS genome during the sequencing run. The total coverage still accumulated as unblocked reads, though short, still map. Sequencing was automatically terminated once each sample reached 50x. The small overshoot in sequenced reads coverage is likely caused by the centrifuge step lagging as reads are not instantly written to disk. C) Stacked area graph illustrating how the proportion of bases mapping to each species changes over time. D) In contrast, the proportion of reads mapping to each species over time doesn’t change significantly. Species and composition as in Figure 2.