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. Author manuscript; available in PMC: 2021 May 30.
Published in final edited form as: Nat Biotechnol. 2020 Nov 30;39(4):442–450. doi: 10.1038/s41587-020-00746-x

Table 2.

Example configurations for different experiment types. “Unblock” causes a read to be ejected from the pore, “proceed” means that a read continues to sequence and serve data through the API for later decisions, “stop receiving” allows the read to continue sequencing with no further data served through the API.

Experiment Type Region of Interest for Alignments Mapping Condition
multi_on multi_off single_on single_off no_map no_seq
Host Depletion Known Host Genome unblock proceed unblock proceed proceed proceed
Targeted Sequencing Known regions from one or more genomes. stop receiving proceed stop receiving unblock proceed proceed
Target Coverage Depth (known sample composition) All known genomes within the sample, tracked for coverage depth. stop receiving proceed stop receiving unblock proceed proceed
Low Abundance Enrichment (unknown sample composition) All genomes within the sample that can be identified as well as those that cannot. stop receiving proceed stop receiving unblock proceed proceed