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. Author manuscript; available in PMC: 2021 Apr 19.
Published in final edited form as: Bioorg Med Chem. 2020 Dec 16;30:115947. doi: 10.1016/j.bmc.2020.115947

Figure 3. Covalent labeling of model peptides with 1.

Figure 3

(a) HPLC analysis of the labeling reaction between Ac-KH6AW peptide (1 μM) and 1 (4 μM) in the presence and absence of Ni2+ (8 μM) at 30 min. (b) Time profile of the reaction between AcKH6AW peptide (1 μM) and 1 (4 μM). (c) Amino acid selectivity of this method. Ac-XH6AW peptides (X = K (Lys), C (Cys), Y (Tyr), S (Ser), T (Thr), E (Glu)) were incubated with Ni2+-complexed 1. The yields at 10 min reaction time were plotted. (d) Summary of the labeling yields at 10 min with the Ac-KAnH6AW peptides (n = 0, 2, 4, 6) in the presence of Ni2+.