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. Author manuscript; available in PMC: 2021 Apr 19.
Published in final edited form as: Anal Chem. 2015 Apr 10;87(8):4144–51. doi: 10.1021/ac504611t

Figure 6. Cell growth and wash away.

Figure 6

(a) Phase contrast time-lapse micrographs (Plan Fluor 10X Ph1) of S. cerevisiae cells, loaded at a density of 5 × 107 cells/mL, and their proliferation until complete pad filling after 15 h (see Video S-1 for full time-lapse movie). (b) Overgrowing cells are washed away by the constant medium flow between the hexagonal pads (bright field micrograph, Plan Fluor 4X). Laminar streamlines minimize cell crosstalk between different pads (see also real-time Video S-2). (c) Cells leaving the cell culturing area exit the chip through the perfusion channel surrounding the culturing chambers (bright field micrograph, Plan Fluor 4X). The parallel chamber arrangement prevents cells from one cell