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. Author manuscript; available in PMC: 2021 Oct 12.
Published in final edited form as: Nat Plants. 2021 Apr 12;7(4):452–467. doi: 10.1038/s41477-021-00889-y

Extended Data Fig. 9. The EVH1 domain of Arabidopsis PP4R3A interacts with meiotic proteins.

Extended Data Fig. 9

a, Yeast two-hybrid assays testing interaction between the PP4R3A EVH1 domain and meiotic proteins. PP4R3A-N indicates the PP4R3A N-terminal region (1-166 aa) containing the EVH1 domain. The yeast co-transformants were grown until OD600 = 1 and spotted on synthetic dropout media (SD) lacking leucine/tryptophan (-LT) and leucine/trptophan/histidine/adenine (-LTHA) for 3 and 5 days. The yeast transformants were grown until OD600 = 1, then diluted 10-, 100- and 1,000-fold in water, and spotted on SD (-LT) and SD (-LTHA) plates to examine growth. b, Venn diagram summarizing yeast two hybrid assays of meiotic proteins that interact with HCR1/PPX1 and the PP4R3A EVH1 domain. c, A schematic model of Arabidopsis PP4 holoenzyme complex that recognizes target protein HEI10 for dephosphorylation via the PP4R3A EVH1 domain and PPX1.