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. Author manuscript; available in PMC: 2021 Oct 12.
Published in final edited form as: Nat Plants. 2021 Apr 12;7(4):452–467. doi: 10.1038/s41477-021-00889-y

Figure 5. Meiotic MLH1 foci are elevated in hcr1 whereas RAD51, ASY1 and ZYP1 immunostaining are unchanged.

Figure 5

a, Representative images of male meiocytes spread and stained with DAPI in wild type (Col) and hcr1-1, at the labeled stages of meiosis. Scale bars=10 μm. b, Representative images of ASY1 (green) and ZYP1 (green) immunostaining of wild type (Col-0) and hcr1-1 male meiocytes at pachytene. Nuclei spreads were also stained with DAPI. Scale bars=10 μM. c, Representative images of ASY1 (green) and RAD51 (red) co-immunostaining on wild type (Col-0) and hcr1-1 male meiocytes during early prophase I. Scale bars=10 μM. d, Quantification of RAD51 foci number per cell in wild type and hcr1-1. e, Representative images of MLH1 (red) immunostaining of male meiocytes at diakinesis stage in wild type, hcr1-1 and meiMIGS-PPX1-PPX2. Cells were also DNA stained with DAPI (blue). Arrows represent MLH1 foci at distal locations on the chromosomes. Scale bars=10 μM. f, Quantification of MLH1 foci number per cell scored at diakinesis stage in wild type (blue), hcr1-1 (red) and meiMIGS-PPX1-PPX2 (red). Scale bars=10 μM. All cytological experiments represent data collected from at least two biological replicates.