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. Author manuscript; available in PMC: 2021 Apr 27.
Published in final edited form as: Nat Struct Mol Biol. 2020 Jul 13;27(8):743–751. doi: 10.1038/s41594-020-0457-x

Extended Data Fig. 6. Structure determination of the +ATP condensin complex.

Extended Data Fig. 6

a, Workflow of the initial data processing of pentameric condensin in the presence of ATP by reference-free ab initio model estimation, combined with focused refinement and representative 2D class averages of the three major classes of the overall complex. b. 2D class averages of open and rod-shaped coiled coils after re-centering and re-extraction of the arm segments (left), and of the ATPase heads after re-centering and re-extraction the ATPase head segments (right) with FSC curves of the cryo-EM density maps.