a, 3D classification of all head particles results in two distinct maps, one with clear density for both coiled coils (open coils, right) and a second one with weak Smc2 coiled coil density (‘single’ coil, left). b, Representative 2D class averages, local resolution as plotted by ResMap52, and FSC curves of the cryo-EM density map of the ‘single’ coil state, as plotted by RELION. c, Representative 2D class averages, local resolution, and FSC curves of the cryo-EM density map of the open coil state.