Table 2. Cryo-EM data collection, refinement and validation statistics of condensin +ATP states.
| +ATP head segment (EMDB-10944) (PDB 6YVD) | +ATP rod-shaped arm segment (EMD-10964) | |
|---|---|---|
| Data collection and processing | ||
| Magnification | 81,000 | 81,000 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e–/Å2) | 45 | 45 |
| Defocus range (μm) | –1.5 ~ –2.5 | –1.5 ~ –2.5 |
| Pixel size (Å) | 1.70 | 1.70 |
| Symmetry imposed | C1 | C1 |
| Initial particle images (no.) | 425,957 | 425,957 |
| Final particle images (no.) | 87,774 | 90,728 |
| Map resolution (Å) FSC threshold | 7.6 at 0.143 FSC threshold | 8.2 at 0.143 FSC threshold |
| Map resolution range (Å) | 6.5 – 10.5 | 7.5 – 11 |
| Refinement | ||
| Initial model used (PDB code) | Homology model derived from 5OQQ, 6QJ1, 6QJ2 |
|
| Model resolution (Å) | 8.4 | |
| FSC threshold | 0.143 | |
| Model resolution range (Å) | n/a | |
| Map sharpening B factor (Å2) | -600 | |
| Model composition | ||
| Non-hydrogen atoms | 8,732 | |
| Protein residues | 1,760 | |
| Ligands | n/a | |
| B factors (Å2) | ||
| Protein | 41.20 | |
| Ligand | n/a | |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.007 | |
| Bond angles (°) | 1.410 | |
| Validation | ||
| MolProbity score | 1.30 | |
| Clashscore | 0.80 | |
| Poor rotamers (%) | 0.00 | |
| Ramachandran plot | ||
| Favored (%) | 89.94 | |
| Allowed (%) | 8.38 | |
| Disallowed (%) | 1.68 |