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. Author manuscript; available in PMC: 2021 Oct 27.
Published in final edited form as: Biochemistry. 2021 Apr 7;60(16):1312–1324. doi: 10.1021/acs.biochem.1c00171

Figure 1. The SHs and ABPP.

Figure 1

(A) The consensus catalytic mechanism of the enzymes from the SH superfamily. (B) Generic structure of an activity-based probe, having three components, i.e. the reactive group or warhead, a linker and a reporter tag. (C) Reaction of a SH enzyme with a fluorophosphonate (FP) activity probe, leading to the formation of an irreversible covalent adduct, thus showing the utility of FP-probes towards ABPP applications.