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. Author manuscript; available in PMC: 2021 Oct 27.
Published in final edited form as: Biochemistry. 2021 Apr 7;60(16):1312–1324. doi: 10.1021/acs.biochem.1c00171

Figure 5. Determining the full complement of active SHs in adult flies.

Figure 5

Heat map plots for the ‘Serine Proteases’, and ‘Metabolic Serine Hydrolases’, showing SH activity signals identified from a LC-MS/MS based ABPP experiment using the SH-directed FP-biotin probe from various tissues and/or anatomical components from adult male and female flies. Data in the heat maps are represented as average peptide counts from three independent biological replicates, and peptide counts above 40 & 100 for the serine proteases and metabolic SHs respectively are colored red. For the head and gut analysis, S = supernatant proteomic fraction, P = pellet proteomic fraction.