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. Author manuscript; available in PMC: 2021 May 3.
Published in final edited form as: Circ Res. 2021 Feb 24;128(9):1258–1275. doi: 10.1161/CIRCRESAHA.120.318688

Figure 5. The CARMN locus is downregulated in human and murine atherosclerosis.

Figure 5

A) Expression level of CARMN and B) Expression level of MMP1, ELN, IL1-beta, CCL2 as FPKM in paired, stable (n=4) and unstable (n=4) human plaque segments retrieved after endarterectomy from 4 symptomatic patients. P value obtained based on the raw read counts using DESeq2. C) Expression of CARMN and miR143/145 in human stable and unstable plaques isolated through endarterectomy (n=4) determined by qRT-PCR. Mann-Whitney was used to assess statistical significance indicated with p values. D) Representative bright field images of in-situ detection of CARMN co-localizing with α-SMA, CD68 or CD45 signal in plaques obtained from carotid artery derived from symptomatic patients at carotid endarterectomy. Pictures were acquired consecutively at 4X, 10X and 40X magnification Scale bar 50μm. E) Representative Haematoxylin & Eosin staining of aortic roots isolated from LDLR-/- mice. Pictures were acquired with 4x and 10X magnification. Scale bar 100μm. F) Expression levels of CARMN and miR143/145 in fatty streak (n=4) and advanced murine plaques (n=11) isolated by Laser Capture Microdissection. Mann-Whitney was used to assess statistical significance indicated with p values.