Skip to main content
. Author manuscript; available in PMC: 2021 May 3.
Published in final edited form as: Circ Res. 2021 Feb 24;128(9):1258–1275. doi: 10.1161/CIRCRESAHA.120.318688

Figure 7. The genetic knock-out of CARMN increased the expression of Lgals3 in plaques developed in the aortic arch of CARMN-/- animals versus wild type controls.

Figure 7

A) Representative EdU staining of proliferating cells in plaques from CARMN+/+ and CARMN-/- animals. Pictures were acquired with 10X magnification. Scale bar 80μm. B) Quantification of proliferating cells in plaques of CARMN+/+ (n=10) and CARMN-/- (n=9) animals. Values are expressed as % of positive nuclei over the total cells counted in the plaque. C) and E) Representative immunostainings and D) and F) quantification of the positive area for αSMA and Lgals3 staining respectively in the plaques of CARMN+/+ (n=10) and CARMN-/- (n=9) animals. Pictures acquired at 10X magnification. Scale bar 200μm. G) Representative picrosirius red staining and H) quantification of collagen content in the plaques of CARMN+/+ (n=10) and CARMN-/-(n=9) animals. The pictures were acquired by polarized light microscopy at 10X magnification. Scale bar 200μm. Student’s t-test was used to assess statistical significance indicated with p values.