| S.
meliloti |
| Rm1021 |
wild
type strain |
(22) |
| ΔhsdR |
Rm1021 strain with hsdR deletion |
(45) |
| SmCreΔhsdR |
Rm1021 cre expression strain with hsdR deletion, tauX:: cre-tetRA (Tcr) |
(45) |
| SmA818 |
strain 2011 cured of megaplasmid
pSymA |
(79) |
| JDSm106 |
Rm1021 bearing tetR-YFP-mChr-parBYp (pJD120)
under control of PtauAB
|
this study |
| MWSm8 |
Rm1021 bearing pMW1 genomically
integrated (Kmr) |
this study |
| MWSm37 |
ΔhsdR bearing pMW28 for constitutive egfp expression (Kmr) |
this study |
| SmCre_exo-IN |
SmCreΔhsdR bearing pIso and Pmin2-loxR-aacC1 integrated up- and downstream
of the exo gene cluster, respectively (Kmr, Gmr) |
this study |
| SmCre_exo-OUT |
SmCreΔhsdR
carrying pIso-exo isolated from pSymB (IPTG
dependent) (Kmr, Specr) |
this study |
| A. tumefaciens |
| C58 |
wild type strain |
(24, 25) |
| C58 exoB |
exhibiting a CFGE-mediated
inactivation of exoB (Gmr) |
(45) |
| exoB/pIso-exo |
C58 exoB carrying pIso-exo (IPTG dependent) (Gmr, Kmr, Specr) |
this study |
| R.
etli |
| CFN42 |
wild type
strain |
(80) |
| S. medicae |
| WSM419 |
wild type strain |
(81) |
| CC169 |
wild type strain |
(82) |
| S.
fredii |
| NGR234 |
wild
type strain |
(83) |
| M. loti |
| MAFF303099 |
wild type strain |
(21) |
| R. leguminosarum |
| Norway |
wild type strain |
(84) |
| M.
extorquens |
| AM1 Δcel
|
strain AM1 deficient in cellulose production |
(85) |
| C. crescentus |
| CB15 N |
wild type strain |
(86) |
| E.
coli |
| DH5α |
used for cloning procedures and plasmid extraction |
(87) |
| S17–1 |
used for conjugation with S. meliloti
|
(88) |
| XL1-Blue |
used
as recipient of pRK2013 (Tcr) |
Stratagene |
| XL1Blue-pRK2013 |
used for triparental matings (Tcr, Kmr) |
this study |
| AB3219 |
used as Clm resistant recipient of pISO-exo (Clmr) |
(89) |
| Plasmids |
| pK/G18mob2 |
suicide vector carrying replication origin
pMB1 |
(39) |
| pK18mobsacB |
suicide vector carrying sacB for
sucrose selection |
(39) |
| pSRK-Gm/Km |
broad-host-range expression vector |
(90) |
| pXG-10 |
carrying replication
origin pSC101* (derivative of pSC101 exhibiting
reduced copy number in E. coli) |
(91) |
| pACYC184 |
carrying replication
origin p15A |
(92) |
| pPHU231 |
broad-host-range expression vector |
(93) |
| pRK2013 |
helper plasmid
for mobilization of nonself-transmissible plasmids |
(94) |
| pLAU44 |
pUC18 derivate
carrying tet operator (tetO) cassettes |
(57) |
| pART |
artificial
mini-replicon based on a pSymA derived repA2B2C2 cassette |
(58) |
| pK18mob2-repABCpSymA
|
pK18mob2 carrying a pSymA derived repA2B2C2 cassette |
this work |
| p42b_p |
pK18mob2 carrying repABCp42b based oriV (R. etli) |
this work |
| p42d_p |
pK18mob2 carrying repABCp42d based oriV (R. etli) |
this work |
| p42e_s |
pK18mob2 carrying a truncated repABC region
derived from p42e (R. etli) |
this work |
| p42f1_p |
pK18mob2 carrying repABCp42f1 based oriV (R. etli) |
this work |
| p42f2_p |
pK18mob2 carrying repABCp42f2 based oriV (R. etli) |
this work |
| pMla_s |
pK18mob2 carrying a truncated repABC region
derived from pMla (M. loti) |
this work |
| pMlb_p |
pK18mob2 carrying repABCpMlb based oriV (M. loti) |
this work |
| pSMED01_p |
pK18mob2 carrying repABCpSMED01 based oriV (S. medicae) |
this work |
| pSMED02_p |
pK18mob2 carrying repABCpSMED02 based oriV (S. medicae) |
this work |
| pNGR234b_p |
pK18mob2
carrying repABCpNGR234b based oriV (S. fredii) |
this work |
| pTi_p |
pK18mob2 carrying repABCpTi based oriV (A. tumefaciens). |
this work |
| pMW1 |
integrative pK18mob2 derivative conferring Kmr
|
this work |
| pMW28 |
integrative
pK18mob2 derivative carrying Pmin2-egfp
|
this work |
| pJD120 |
pK18mobsacB derivative, integration of tetR-YFP-mChr-parBYp under control of PtauAB
|
this work |
| pJD211 |
pK18mob2
derivative carrying Pmin2-loxR-aacC1
|
this work |
| pPHU231-egfp |
pPHU231
derivative carrying a constitutive egfp cassette |
this work |
| pABCa-egfp |
pABCa carrying a constitutive egfp cassette |
this work |
| pABCa-egfp-expR |
pABCa carrying constitutively expressed egfp-expR
|
this work |
| pABCa-tetO |
pABCa carrying a tetO array |
this work |
| pABCb-egfp |
pABCb
carrying a constitutive egfp cassette |
this work |
| pABCb-egfp-expR |
pABCb carrying constitutively expressed egfp-expR
|
this work |
| pABCc-egfp-expR |
pABCc carrying constitutively expressed egfp-expR
|
this work |
| pABCc-parSYp
|
pABCc carrying parSYp
|
this work |
| pABCc-mob-egfp |
pABCc-mob carrying a constitutive egfp cassette |
this work |
| pABC1-egfp |
pABC1 carrying a constitutive egfp cassette |
this work |
| pABC1-egfp-expR |
pABC1 carrying constitutively
expressed egfp-expR
|
this work |
| pABC1-egfp_f |
pABC1 carrying a forwardly integrated SD-egfp fragment |
this work |
| pABC1-egfp_r |
pABC1 carrying a reversely
integrated SD-egfp fragment |
this work |
| pABC1-T2-egfp_f |
pABC1-T2 carrying a forwardly integrated SD-egfp fragment |
this work |
| pABC1-T2-egfp_r |
pABC1-T2 carrying a
reversely integrated SD-egfp fragment |
this work |
| pABC1-T3-egfp_f |
pABC1-T3 carrying a forwardly integrated SD-egfp fragment |
this work |
| pABC1-T3-egfp_r |
pABC1-T3 carrying a reversely integrated SD-egfp fragment |
this work |
| pABC2-egfp |
pABC2 carrying a constitutive egfp cassette |
this work |
| pABC2-egfp-expR |
pABC2 carrying constitutively expressed egfp-expR
|
this work |
| pABC3-egfp |
pABC3 carrying a constitutive egfp cassette |
this work |
| pABC4-egfp |
pABC4
carrying a constitutive egfp cassette |
this work |
| pABC4-egfp-expR |
pABC4 carrying constitutively expressed egfp-expR
|
this work |
| pABCb-lacI |
pABCb
carrying lacI for constitutive
LacI expression |
this work |
| pMlb_lacO |
pK18mob2 carrying repABCpMlb bearing
a lacO cassette at the predicted transcription start
site |
this work |
| pIso |
ABC cloning vector integrating upstream of the exo gene cluster on pSymB |
this work |
| pIso-exo |
isolated ABC cloning vector carrying
the exo gene cluster (replicative upon IPTG supplementation) |
this work |
| pABC Library Plasmids Carrying
Module Parts |
| pLoriVSm |
pK18mob2
derivative, unloaded library vector for oriVSm parts |
this work |
| pLsynTer-MCS |
pK18mob2
derivative, unloaded library vector for synTer-MCS
parts |
this work |
| pLoriT |
pK18mob2 derivative, unloaded library vector for oriT parts |
this work |
| pLAR |
pK18mob2
derivative, unloaded library vector for AR parts |
this
work |
| pLoriV-42b |
pLoriVSm derivative
providing the oriVp42b region (R. etli) |
this work |
| pLoriV-42d |
pLoriVSm derivative providing the oriVp42d region (R.
etli)
|
this work |
| pLoriV-42e |
pLoriVSm derivative providing the oriVp42e region (R. etli) |
this work |
| pLoriV-42f1 |
pLoriVSm derivative providing
the repA1B1C1 based oriVp42f region (R. etli) |
this work |
| pLoriV-42f2 |
pLoriVSm derivative providing
the repA2B2C2 based oriVp42f region (R. etli) |
this work |
| pLoriV-Mla |
pLoriVSm derivative providing
the oriVpMla region (M. loti) |
this work |
| pLoriV-Mlb |
pLoriVSm derivative providing the oriVpMlb region (M. loti) |
this work |
| pLoriV-Ti |
pLoriVSm derivative providing
the oriVpTi region (A. tumefaciens) |
this work |
| pLsynTer-1 |
pLsynTer-MCS derivative providing synTer1-MCS |
this work |
| pLsynTer-2 |
pLsynTer-MCS derivative providing synTer2-MCS |
this work |
| pLsynTer-3 |
pLsynTer-MCS derivative providing synTer3-MCS |
this work |
| pLsynTer-1-lox |
pLsynTer-MCS derivative providing synTer1-MCS-loxL
|
this work |
| pLsynTer-2-lox |
pLsynTer-MCS derivative
providing synTer2-MCS-loxL
|
this work |
| pLsynTer-3-lox |
pLsynTer-MCS derivative providing synTer3-MCS-loxL
|
this work |
| pLoriT-1 |
pLoriT
derivative providing a mob site |
this
work |
| pLoriT-1-lox |
pLoriT derivative providing mob-loxR
|
this work |
| pLoriT-1-loxT |
pLoriT derivative providing mob-loxR-rrnBT2
|
this work |
| pLAR-Km |
pLAR derivative providing a kanamycin resistance cassette |
this work |
| pLAR-Spec |
pLAR derivative providing a spectinomycin resistance
cassette |
this work |
| pLAR-Tc |
pLAR derivative providing a
tetracyclin resistance cassette |
this work |
| pLAR-Hyg |
pLAR derivative providing a hygromycin resistance cassette |
this work |
| pLAR-Tmp |
pLAR derivative providing a trimethoprim resistance
cassette |
this work |
| pLAR-Gm |
pLAR derivative providing a
gentamicin resistance cassette |
this work |
