Extended Data Fig. 10. In vitro effect of BYL719 and rapamycin on fibroblasts from PIK3CA^CAGG-CreER mice.

a, Skin fibroblasts from PIK3CA^WT and PIK3CA^CAGG-CreER mice were isolated and exposed to vehicle or increasing concentrations of BYL719 or rapamycin for 24 h. b, Quantification. White column, without 4-OHT; black column, with 4-OHT. All data are shown as mean ± s.e.m. ANOVA followed by Tukey– Kramer test (two-tailed). Before versus after Cre induction with 4-OHT, ***P < 0.001. BYL719 or rapamycin exposure compared with cells treated with vehicle, ##P < 0.01 and ###P < 0.001.